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The Wheel Of Time Epub Free 392
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Extracts containing only endogenous proteins were incubated overnight with the preferred antibody at 4C on a rotating wheel in presence of Ethidium Bromide. Next day the lysate was transferred to SureBeads Protein G Magnetic beads for 2 hours at 4C on a rotating wheel. Samples were washed three times using WCE buffer and a SureBeads Magnetic Rack (Bio-Rad).
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